Environmental DNA, or eDNA, is genetic material that organisms release into their surroundings.
For great crested newts, ecologists can collect pond-water samples and arrange laboratory analysis to investigate whether the species is present.
Under established Natural England protocols, the commonly accepted eDNA sampling period is 15 April to 30 June.
Sampling must follow an appropriate protocol, and results require professional interpretation.
A positive result indicates that great crested newt DNA has been detected. However, eDNA testing does not provide a population size estimate.
A negative result should also be interpreted in light of sampling conditions, the relevant protocol and the wider ecological evidence.
If the assessment is needed to support a particular licensing application, confirm the applicable sampling, laboratory and surveyor requirements before undertaking work.